Hideyoshi Yoshino* and Kohei Urano**

*Kanagwa Environmental Research Center, Division of Environmental Engineering
(842, Nakaharashimojyuku, Hiratuka-city, Kanagawa 254 Japan)
**Yokohama National University, Laboratory of Safety and Environmental Engineering

Abstract

There are many kinds mutagenic of chemicals found in the fly ash that comes from municipal incinerators. In this study, the solvent extraction method was investigated for the Ames mutagenicity test of fly ash. Tested solvents were hexane, ethyl acetate, aceton, ethanol, dimethylsulfoxide (DMSO), methanol and distilled water. Higher extraction efficiencies of the mutagenic substances were obtained by DMSO for most of the fly ash samples, and DMSO could be used directly in the Ames test. The mutagenic substances could be recovered almost completely using 2 times the extraction for 10 minutes with 6ml of DMSO per 1g of fly ash. Consequently, the extraction method shown in Fig.10 is recommended for the Ames mutagenicity test of fly ash from incinerators. Higher mutagenicity was observed in the tests using S 9 mix by this extraction method.

Key words: municipal waste, mutation, ames test, extraction, fly ash