Yoshiro Ono*, Saeko Kanoh**, Kentaro Aoi**, Masato Yamada***, Tsutomu Imaoka**** and Osami Kawara*

* Department of Environmental & Civil Engineering, University of Okayama
** Graduate School of Environmental Systems, University of Okayama
*** Department of Waste Management Engineering, National Institute of Public Health
**** Hiroshima Institute of Technology

+ Correspondence should be addressed to Yoshiro Ono:
(3-1-1 Tushima-naka Okayama-city 700-8530 Japan)

Abstract

Risk evaluation will be needed for waste materials, such as fly and bottom ash, and leachate (including hazardous substances) . Bio-tests can be available to monitor the total toxicity of waste samples. Because of the contamination of various substances in the waste materials, it is too costly to identify harmful substances. The bio-tests are expected to determine the total toxicity within a short experimental period. In this research, genotoxicity using bacterial assay, umu-tests and estrogenic cell growth activity using the human breast cancer cells, MCF-7, were applied to elute from ash and leachate samples. Some samples were found to have toxic activity, and the estrogenic cell growth concentration was 10-8, lower than that of the genotoxicity.

Key words: fly ash, bottom ash, leachate, estrogenic cell growth activity, genotoxicity